Cloning vectors :- A cloning vector is a genome that can accept the target DNA and increase the number of copies through its own autonomous replication. It can be a plasmid, a bacteriophage, or yeast artificial chromosome (YAC). Cloning vectors usually are selected on the basis of differences in their capacity for the size of the insert DNA. Many types of vector and host cells are employed in cloning experiments. The choice of vector depends on the size of the insert to be cloned and the purpose of the experiment . Plasmids and lambda bacteriophage vectors are used for cloning small inserts of up to a few kilobases of DNA; cosmid vectors, on the other hand, can replicate efficiently with up to about 50kb of DNA insert; bacterial artificial chromosomes (BACs) and yeast artificial chromosomes (YACs) are capable of replicating significantly larger inserts of up to about 500kb of DNA (BACs) and more than 1000kb of DNA (YACs). .Experience showed that whereas YACs were capable o...
